This is because Studio One by default will auto-enable input monitoring when enabling recording.

Pro User Tip: With Virtual Loopback enabled in Universal Control, muting the Virtual track in Studio One before enabling recording helps avoid a feedback loop.

Mute this Virtual Input 1 + 2 track, enable recording, and disable input monitoring.

Change the existing Stereo track source to 'Virtual Input 1 + 2' and change the track name to the same.

Rename this Input 'Virtual Input 1 + 2'.

Map L to Virtual in 1, R to Virtual in 2.

Click Studio One > Options > Audio Setup > Song Setup.

New Song > Interfaces > ioStation 24c template.

To set up Studio One I/O with a New Song open using the ioStation 24c template,:

Set Audio output to 'Main Out 1/2 (ioStation 24c)'.

Set Audio input to 'Mic/Inst/Line In 1/2 (ioStation 24c)'.To set the ioStation 24c as your audio device in Skype: Select 'Virtual' in the Loopback dropdown box.Note: Please first ensure proper ioStation 24c setup in Windows and Studio One: This feature allows the user to be able to record Skype sessions in Studio One or other DAW, along with any computer audio playback. The ioStation 24c has a Loopback feature available to Windows users, outlined in section 3.1.2 of the ioStation24c manual.

Skype sign in loop